Mouse Secondary Antibodies
Biotium ALK (Anaplastic Lymphoma Kinase) / CD246(ALK/3218R), CF568 conjugate, 0.1mg/mL
The wild-type anaplastic lymphoma kinase (ALK) protein is a 200 kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes). ALK has been found to be rearranged, mutated, or amplified in a series of tumors. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation, encodes part of the nucleolar phosphoprotein nucleophosmin (NPM), joined to the entire cytoplasmic portion of ALK. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80 kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein, producing a c
Biotium Tyrosinase (Melanoma Marker)(rOCA1/812), CF740 conjugate, 0.1mg/mL
Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3, 4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes
Biotium MCM6 (Proliferation Marker)(MCM6/3000), CF740 conjugate, 0.1mg/mL
The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundanc
Abcam Anti-MUC16 antibody (OV185:1)
Anti-MUC16 antibody (OV185:1)
The product is subject to the following: Abcam Restricted Use Statement
Biotium p73 (Tumor Suppressor)(P73/2531), CF488A conjugate, 0.1mg/mL
p73 shares considerable sequence similarity with the widely-studied tumor suppressor gene p53. The p73 gene maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, unlike p53, p73 does not appear to be activated by DNA damaging agents.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium SOX4 (Master Regulator of Epithelial-Mesenchymal Transition) (SOX4/2540), CF488A conjugate, 0.1mg/mL
SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF-β-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival in vitro and for primary tumor growth and metastasis in vivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates